MacroSep™ BIO-Gold packings are based on ultra-high purity spherical silica, state-of-the-art high density bonding and full endcapping produces the separation or purification of high molecular weight bio compounds such as proteins and peptides. Significant improvements in acidic and alkaline resistance has been achieved, so MacroSep BIO-Gold materials can be used for extended periods of time in acidic mobile phase condition and rinsed for recovery with NaOH containing buffer. MacroSep BIO-Gold columns are manufactured utilizing tight process control of the silica, bonding and column packing processes. The reproducible column packing method control provides exceptional efficiency, symmetry and reproducibility.